Waste water treatment using Moringa oleifera: Drying, extraction and response surface methodology optimisation studies

This project will be focused on the impact of hot air drying temperatures and solid to solvent ratios to the preparation of the Moringa oleifera seeds as the natural coagulant to treat dye wastewater.

Conventional coagulants such as aluminum sulphate and ferric sulphate have proved to be harmful to human health as it may cause neurological diseases like percentile dementia and Alzheimer. This issue has led to the use of natural coagulants which are environmental friendly and not harmful to humans. In this study, Moringa Oleifera seeds will be used to study the preparation of the natural coagulant and further use it in the treatment of dye wastewater. There are 2 important steps in preparing the natural coagulants which are drying of the raw materials and protein extraction. Oven drying will be performed on Moringa Oleifera seeds at 40C, 50C and 60C and the drying data will be collected. This data will then be fitted into thin layer kinetic models to obtain the most suitable drying temperature and its respective drying time. In the next step, the plant-based natural coagulant which has been dried at the optimum temperature and time will be used for protein extraction. The solid to solvent ratio of 0.5:100, 1:100, 2:100 and 5: 100 (g of Moringa oleifera seeds: ml of distilled water) will be used to extract the protein by using a domestic blender for 2 minutes. The best solid to solvent ratio will be selected based on the highest protein content using Bradford method. Using the best solid to solvent ratio solution, jar test will be performed. Central Composite Design will be used to optimize the performance of MO seeds by varying process parameters such as coagulant dosage, pH and initial concentration of dye wastewater.

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